Caspase-1, human recombinant

Catalog number
1081-100
Name
Caspase-1, human recombinant
Size
100 units
Price
414.00 EUR
Supplier

Details

Target
Caspase-1
Background
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.
Concentration
N/A
Molecular weight
large (20 kD) and small (10 kD) subunitsk
Synonym
Caspase-1
Other name
Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, IL-1 beta-converting enzyme, p45
NCBI gene number
834
NCBI gene
CASP1
Gene source
Human
NCBI number
NP_150634.1
Recombinant
Yes
Source
E. coli.
Purification
N/A
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
> 5000 units/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Semi-Dry powder
Physical properties
Lyophilized powder
How to reconstitute
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants