Target of this antibody:
Phospho-Erk1/2 (p44/p42 MAPK)
Alternative names of antibody target:
MAPK1, p38, ERK , P42MAPK, ERK2 , p40 , PRKM1 , p41mapk , PRKM2, MAPK2 , p42-MAPK, p41 , ERK-2, ERT1
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs throμgh phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic phospho-peptide surrounding Thr202/Tyr204 of human P44MAP kinase
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4µg/ml), immunoprecipitation(10-20 µg/ml),and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes Phosphorylated p42 and p44 (Erk1 and Erk2).
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.