anti-EGLN1 Antibody

Catalog number
6151-100
Name
anti-EGLN1 Antibody
Size
EGLN1 Antibody
Price
414.00 EUR
Supplier

Details

Target of this antibody:
EGLN1
Alternative names of antibody target:
EGLN1, DKFZp 61F179, ECYT3, HIFPH2, HPH2, PHD2, SM­20, SM20, ZMYND6.
Background information:
PHD1 (Egln2), PHD­2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle. The mRNA levels of PHD are upregulated by HIF through the hypoxia response element under low oxygen conditions. These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Recombinant protein of human EGLN1
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Recommended usage:
Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps