Target of this antibody:
Alternative names of antibody target:
brain-derived neurotrophic factor, brain derived neurotrophic factor
Human Brain-Derived Neurotrophic Factor (BDGF) is a potent neurotrophic factor that supports the growth and survivability of nerve and glial cells. BDNF protein is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions. The antibody recognizes the mature form of BDNF at 14 kDa.
Clonality of antibody:
This antibody reacts with:
Recombinant human BDNF
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-human BDNF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. Recombinant human BDNF (Cat.# 4004-10) can be used as a positive control.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.