Target of this antibody:
Alternative names of antibody target:
PDPK-1 , PDPK1 , PDK1 , hPDK1 , MGC35290 , MGC20087 , PkB-like
PDK1 (Phosphoinositide-dependent protein kinase 1) plays an important role in cell signaling pathways and is involved in regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 542 of human PDK1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~60 kDa PDK1 of human, mouse, and rat origins. A ~33 kDa cleavage fragment can also been recognized. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.