Caspase-2, human recombinant

Catalog number
1082-25
Name
Caspase-2, human recombinant
Size
25 units
Price
262.00 EUR
Supplier

Details

Target
Caspase-2
Background
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA (Cat. #1072-200) or Ac-VDVAD-AFC (1071-200).
Concentration
N/A
Molecular weight
two large (19 kDa) and two small (12 kDa) subunits
Synonym
Caspase-2
Other name
Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1
NCBI gene number
835
NCBI gene
CASP2
Gene source
Human
NCBI number
P42575
Recombinant
Yes
Source
E. coli.
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
10,000 – 13,000 units/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized powder
How to reconstitute
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants