TNF-alpha, rat recombinant

Catalog number
1052-1000
Name
TNF-alpha, rat recombinant
Size
1 mg
Price
3149.00 EUR
Supplier

Details

Target
TNF-alpha
Background
Tumor Necrosis Factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-α is a 17 kDa protein containing 157 amino acid residues.
Concentration
N/A
Molecular weight
17.0 kDa
Synonym
TNF, Tumor necrosis factor- alpha
Other name
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
NCBI gene number
24835
NCBI gene
TNFA
Gene source
Rat
NCBI number
P16599.1
Recombinant
Yes
Source
E. coli.
Purification
≥98%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.05 ng/ml, corresponding to a specific activity of> 2 x 10⁷ units/mg.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
gel pack
Physical appearance
Lyophilized powder
Physical properties
Sterile filtered and lyophilized from 1X PBS
How to reconstitute
We recommend a quick spin followed by reconstitution in H₂O to a concentration of 0.1 – 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
The TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus
Additional source
Recombinants or rec. proteins
Group
recombinants
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.