anti-HA-FITC Antibody (Clone 2C16)

Catalog number
3996F-100
Name
anti-HA-FITC Antibody (Clone 2C16)
Size
HA-FITC Antibody (Clone 2C16)
Price
360.00 EUR
Supplier

Details

Target of this antibody:
HA-Tag-FITC
Alternative names of antibody target:
GRIN2A , hNR2A , NMDAR2A , NR2A
Background information:
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG2b
Clone:
2C16
This antibody reacts with:
All species
Immunogene sequence:
Synthetic peptide of 76-111 of X47 hemagglutanin 1
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.2 mg/ml) mouse anti-HA-FITC monoclonal antibody (>90% pure) in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Immunofluorescence
Recommended usage:
The Anti-HA-FITC antibody can be used for one-step localization of recombinant HA-tagged proteins by standard immunofluorescence methods (2-10 µg/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody
French translation
anticorps