Target of this antibody:
Alternative names of antibody target:
MSH-6, MSH 6, DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTMBP, GTBP , mutS (E. coli) homolog 6, HNPCC 5, HNPCC5, MSH 6, MSH6
The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage. MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex. Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world. Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human MSH6
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µL affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 to 1:2000). However, the optimal concentrations should be determined individually. TK6 cell lysate can be used as a positive control. The antibody recognizes human MSH6. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.