Target of this antibody:
Alternative names of antibody target:
Methyl-CpG-binding domain protein 2
The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms, MBD2a and MBD2b. MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 164 of rat MBD2a
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti- MBD2a polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3232BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.