Target of this antibody:
Alternative names of antibody target:
V5-tag, anti-V5-tag, V5-tag antibody,V5 antibody
V5-tag is a 14 amino acid peptide derived from the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5-tagged protein expression system is preferably used in various laboratories. Hence, the anti-V5-Tag antibody is a useful tool for monitoring of the V5-tagged protein.
Clonality of antibody:
This antibody reacts with:
A synthetic V5-Tag peptide GKPIPNPLLGLDST conjμgated to KLH.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-V5-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.