anti-Akt/PKB Antibody

Catalog number
3247-100
Name
anti-Akt/PKB Antibody
Size
Akt/PKB Antibody
Price
360.00 EUR
Supplier

Details

Target of this antibody:
Akt/PKB
Alternative names of antibody target:
PKB, RAC, Akt 1, Akt 2, Akt 3
Background information:
Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat, bovine
Immunogene sequence:
Synthetic peptide corresponding to residue surrounding amino acid 80 of human Akt
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Akt polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot,Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blotting (0.5-4 µg/ml), in immunoprecipitation (10-40 µg/ml), and Immunohistochemistry (10-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Akt1, Akt2 and Akt3.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps