Target of this antibody:
Alternative names of antibody target:
Lysine-specific demethylase 3A
JMJD1A (Jumonji domain containing 1A), belongs to the JHDM2 histone demethylase family. Histone demethylase specifically demethylates 'Lys-9' of histone H3, thus play a central role in histone code. JMJD1A is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 that are required for proper packaging and condensation of sperm chromatin.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 1181 of human JMJD1A
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-JMJD1A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 3273BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.