Target of this antibody:
Alternative names of antibody target:
NIF1, NIF-1, Nuclear Receptor Coactivator-Interacting Factor 1, ZNF335, Zinc Finger 335
NRC-Interacting Factor 1 (NIF1) interacts with the nuclear receptor transcriptional coactivator NRC and enhances ligand-dependent transcriptional activation by nuclear hormone receptors as well as c-Fos and c-Jun. NIF1 may function to influence chromatin structure.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human NIF1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa and NIH3T3 cell lysates can be used as positive controls. However, the optimal concentrations should be determined individually. The antibody recognizes the NIF1 of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.