Target of this antibody:
Alternative names of antibody target:
APG12 , APG12L , ATG12 , HAPG12
Autophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) throμgh phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjμgate with ATG5; this conjμgate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjμgate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 21 of mouse APG12
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
0.5 mg/ml affinity purified rabbit anti-APG12 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.