2-NBDG Glucose Uptake Assay Kit (Cell-Based)

Catalog number
K682-50
Name
2-NBDG Glucose Uptake Assay Kit (Cell-Based)
Size
50 assays
Price
430.00 EUR
Supplier

Details

Kit's description
Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolisms and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose) is a fluorescent deoxyglucose analog that can be taken up by cells through glucose transporters. However, 2-NBDG cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy to use non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors etc.
Highlights
• Detection method: FACS (488 nm excitation laser) and fluorescent microscope (excitation range 420nm:495nm). • Application: Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. Dual:staining of glucose transporters and glucose uptake. Analysis of glucose metabolism and cell signaling in various cell types. Screening of anti:diabetic compounds
Kit's benefits
• Easy-to-use • Non-radioactive • Image and accurately measure glucose uptake in cultured cells in response to insulin, growth factors etc.
Category
Metabolism, Glucose uptake assay
Kit's other name
Glucose uptake assay kit, Cell based Glucose uptake assay, Glucose uptake assay, Cell based Glucose uptake assay kit,
Contents
• Analysis Buffer (50X) • 2-NBDG Reagent (100X) • Glucose Uptake Enhancer • Phloretin (100X)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
The most sensitive FACS kit
Applications
• Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc. • Dual-staining of glucose transporters and glucose uptake • Analysis of glucose metabolism and cell signaling in various cell types • Screening of anti-diabetic compounds
Samples tested
Adherent or suspension cells
Test
Biovision supplies other types of Assays as 1.
Description
For cells, cell lines and tissues in culture till half confluency.