Target of this antibody:
Alternative names of antibody target:
TRIP1, TRIP-1, Thyroid Receptor Interactor 1, TBP10, Tat-binding protein homolog 10, PSMC5, Proteasome (prosome, macropain) 26S Subunit, ATPase, 5, p45, p45/Sμg, Sμg1, S8
Thyroid receptor interacting protein 1 (TRIP1) was originally identified as a protein that interacts with the thyroid receptor and is homologous to the yeast SUG1 protein, a transcriptional coactivator. It was later identified as an ATPase subunit of the 26S proteasome, a multicatalytic proteinase complex, and has also been shown to associate with nuclear receptors and transcription factors. TRIP1/SUG1 may function to regulate transcription factor abundance by targeting them for proteasome degradation.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human TRIP1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 – 1:2500). However, the optimal concentrations should be determined individually. HeLa and NIH3T3 cell lysates can be used as positive controls. The antibody recognizes the TRIP1(Sμg1) of human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.