FGF-2/FGF-basic, human recombinant

Catalog number
4037-10
Name
FGF-2/FGF-basic, human recombinant
Size
10 μg
Price
137.00 EUR
Supplier

Details

Target
FGF-2
Background
FGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Concentration
N/A
Molecular weight
Human FGF-2 is a 17.2 kDa recombinant protein
Synonym
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor
Other name
FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
NCBI gene number
2247
NCBI gene
FGF-2
Gene source
Human
NCBI number
P09038
Recombinant
Yes
Source
E. coli
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED₅₀ of human FGF-2 is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 3 ng/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Recombinant FGF-2 is available as a lyophilized powder
Physical properties
FGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl
How to reconstitute
Reconstitute human FGF-2 in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants