Active Sonic Hedgehog (SHH), human recombinant

Catalog number
4010-25
Name
Active Sonic Hedgehog (SHH), human recombinant
Size
25 μg
Price
251.00 EUR
Supplier

Details

Target
Sonic Hedgehog (SHH)
Background
Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.
Concentration
N/A
Molecular weight
19.697kDa
Synonym
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Other name
SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
NCBI gene number
6469
NCBI gene
SHH
Gene source
Human
NCBI number
Q15465
Recombinant
Yes
Source
E. coli
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
Human recombinant Sonic Hedgehog is biologically active as compared to a standard. The ED₅₀, measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 fibroblasts, is 750ng/ml.
Reesults
750ng/ml.
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5.
How to reconstitute
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants