anti-NFkB p65 Antibody (Clone 2A12A7)

Catalog number
3012-100
Name
anti-NFkB p65 Antibody (Clone 2A12A7)
Size
NFkB p65 Antibody (Clone 2A12A7)
Price
403.00 EUR
Supplier

Details

Target of this antibody:
NFkB p65
Alternative names of antibody target:
RELA, NFKB3, MGC131774
Background information:
NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
Clone:
2A12A7
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide from human NFkB
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (200 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. A431 cells can be used as a positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
French translation
anticorps