Akt Negative Control Cell Lysate

Catalog number
7035-1
Name
Akt Negative Control Cell Lysate
Size
1 mg
Price
295.00 EUR
Supplier

Details

Highlights
• CONCENTRATION- 4 mg/ml
Description
Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three volume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min. Collect the cell lysate. Extract the pellet again with one volume Kinase Extraction Buffer. Combine the cell lysate together, determine protein concentration. The cell lysate can be used as a negative control for Akt activity assays, in conjunction with the use of BioVision’s Akt Activity Assay Kit (K435-40). We recommend using 100 µg per assay.
Category
Akt
Storage condition
-70°C
Shipping condition
dry ice
Maximum time for storage
6 months
Additional description
For cells, cell lines and tissues in culture till half confluency. Isotype or positive controls by peptides, antibodies and deactivated samples. Negative control peptides or isotypes available of the different species.