Target of this antibody:
Alternative names of antibody target:
CASP-2, NEDD-2,NEDD2, ICH1, ICH-1L/1S, ICH-1L, EC 22.214.171.124
Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 417 of mouse caspase-2
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
30 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Western blotting (1-2 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.