UIM-UBA Fusion Protein

Catalog number
6571-250
Name
UIM-UBA Fusion Protein
Size
250 μl
Price
273.00 EUR
Supplier

Details

Highlights
Salient Features: Source: E.Coli. Purity: ≥95% by SDS-PAGE analysis. Molecular weight: ~13.3 kDa. Formulation: 250 µl of 0.4 mg/ml in PBS containing 0.1% azide. Applications: • As negative control for biotin UIM-UBA in detecting polyubiquitinated proteins in cell lysate/tissue homogenate using Dot blot.
Description
Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Different types of polyubiquitin chains can form, depending on the internal lysine residue used for this conjugation. These polyubiquitin chains further can attach to proteins post-translationally and aid in numerous downstream activities like proteasome-mediated proteolysis, autophagy, DNA damage tolerance, inflammation, apoptosis, signal transduction etc. Several classes of Ubiquitin interacting proteins help in mediating these downstream effects. Ubiquitin Interacting Motifs (UIM) and Ubiquitin Associated Domains (UBA) are two large classes of such protein domains which strongly interact with polyubiquitin chains. We have developed an UIM-UBA chimeric protein from the UIM and UBA domains. Both these proteins are well-characterized for their high-affinity interaction with different types of polyubiquitin chains, but not with monomeric ubiquitin.
Category
Ubiquitin
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months
Additional description
Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.