Target of this antibody:
Alternative names of antibody target:
FOX H1, FOXH 1, Forkhead box H1, Forkhead box protein H1,Forkhead activin signal transducer 1, Forkhead activin Signal Transducer 2, FAST-1, FAST1
FoxH1 (also known as Forkhead box H1 or FAST-1) is a transcription factor that shares close homology with Xenopus FAST-1. FoxH1 binds to SMAD2/4 and activates activin response throμgh a DNA binding motif region that consist of TGT(G/T)(T/G)ATT sequence. FoxH1 binding to Smad2/Smad4/FAST1 forms a transcriptionally complex structure that can induced TGFβ signal transduction.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 55 of mouse FoxH1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~44kDa of FoxH1 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.