anti-Anti-Mouse IgG, HRP-Linked Antibody

Catalog number
6402-05
Name
anti-Anti-Mouse IgG, HRP-Linked Antibody
Size
Anti-Mouse IgG, HRP-Linked Antibody
Price
295.00 EUR
Supplier

Details

Target of this antibody:
Anti-Mouse IgG-HRP
Alternative names of antibody target:
Anti-Mouse IgG, HRP-Linked Antibody, HRP linked secondary antibody
Background information:
Rabbit anti-mouse IgG (H+L), horseradish Peroxidase (HPR) conjμgated. This antibody has been optimized as secondary antibody in chemiluminescent Western blotting applications
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Mouse
Immunogene sequence:
N/A
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
0.5 ml in 0.01 M sodium phosphate, 0.15 M NaCl, 1 mg/ml BSA, 25% glycerol, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry, ELISA
Recommended usage:
Western blotting: 1:2,000-1:100,000. Enzyme immunoassays: 1:2,000-1:100,000. Immunohistochemistry: 1:200-1:2,000. The optimal conditions should be determined individually.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation
HRP Conjugated
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Additional isotype
IgG
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps