anti-BMI 1 Antibody

Catalog number
6150-100
Name
anti-BMI 1 Antibody
Size
BMI 1 Antibody
Price
414.00 EUR
Supplier

Details

Target of this antibody:
BMI 1
Alternative names of antibody target:
RNF51, PCGF4
Background information:
The polycomb group (PcG) of proteins contributes to the maintenance of cell identity, stem cell self-­renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long term gene silencing through epigenetic chromatin modifications. The first complex, EED­EZH2, is recruited to genes by DNA­binding transcription factors and methylates histone H3 on Lys27. This histone methyl­transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex. Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119. Bmi1 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16 INK4A and p19 ARF genes and is required for maintenance of adult hematopoietic and neural stem cells.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Recombinant protein of human BMI1
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunohistochemistry
Recommended usage:
Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps