Target of this antibody:
Alternative names of antibody target:
CASP-5, ICH-3 protease, TY protease, ICH3
Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-5 is also known as Ich-3 and ICErel-III and can proteolytically processed by granzyme B. Over expression of caspase-5 induces apoptosis in Rat-1 and HeLa cells, which can be inhibited by CrmA and Bcl-2.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat, monkey, hamster, bovine
Synthetic peptide surrounding amino acids 162 of human caspase-5
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-5 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4ag/ml). However, the optimal conditions should be determined individually. The affinity purified antibody preferentially detects the intermediate (32 kDa) and the fully (20 kDa) cleaved caspase-5.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.