Target of this antibody:
Alternative names of antibody target:
AT complementation group A, AT complementation group C, AT complementation group D, AT complementation group E , ATE, ATDC
The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. All of the members of the PIK-related kinases are also larger than 270 kDa. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT), which is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide from human Ataxia Telangiectasia Mutated (ATM)
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (1:500-2000) and immunoprecipitation. However, the optimal concentrations should be determined individually. Human L-40 cell lysate can be used as a positive control for Western blotting. The antibody recognizes ATM in samples from human and mouse origins. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.