Target of this antibody:
Alternative names of antibody target:
Adipocyte determination- and differentiation-dependent factor 1, ADD1, Sterol regulatory element-binding transcription factor 1
SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors th at regulates cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. SREBP proteins undergo proteolytic cleavage in response to conditions of low cellular sterol. Upon activation of SREBP 1, a soluble NH2 cleavage fragment translocate to the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 318 of rat SREBP1
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-SREBP1polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide (Cat.# 3961BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.