Epidermal Growth Factor (EGF), human recombinant

Catalog number
4022-500
Name
Epidermal Growth Factor (EGF), human recombinant
Size
500 μg
Price
257.00 EUR
Supplier

Details

Target
EGF
Background
Human epidermal growth factor (EGF) is also known as HOMG4 and URG, and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk. Recombinant human EGF is a 6.2 kDa protein containing 53 amino acid residues. This recombinant human EGF has an N-terminal His-tag preceding the 53 amino acid sequence (MW = 8.5 kDa)
Concentration
N/A
Molecular weight
Human EGF is a 6.2 kDa recombinant protein
Synonym
Urogastrone, URG, EGF, epidermal growth factor
Other name
Urogastrone, URG, EGF, epidermal growth factor
NCBI gene number
UniGeneHs.419815
NCBI gene
EGF
Gene source
Human
NCBI number
Q6QBS2
Recombinant
Yes
Source
E. coli
Purification
≥98%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
The ED₅₀ of human EGF is determined by the dose-dependent stimulation of BALB/c 3T3 cells is 5.92-10.06 ng/ml.
Reesults
5.92-10.06 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Recombinant EGF is available as a lyophilized powder
Physical properties
EGF protein is lyophilized with no additives
How to reconstitute
Reconstitute the human EGF in H₂O to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants