Target of this antibody:
Alternative names of antibody target:
TCF3 (E2A) fusion partner, isoform CRA_b (Uncharacterized protein)
Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play important roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'.Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 556 of rat TCF3/E2A
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti- TCF3/E2A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western Blot analysis (1 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3090BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.