Target of this antibody:
Alternative names of antibody target:
KLF4 belongs to the erythroid Kruppel-like factor (EKLF) family that is involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. KLF4 can act as both a repressor and activator of transcription. KLF4 regulates the expression of key transcription factors during embryonic development and is important in maintaining embryonic stem cells. It also contributes to the down-regulation of p53/TP53 transcription.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 27 of rat KLF4
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
200 µg (0.5 mg/ml) affinity purified rabbit anti-KLF4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western Blot analysis (1 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3089BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.