Active Porcine Calpain 1

Catalog number
1138-100
Name
Active Porcine Calpain 1
Size
100 μg
Price
387.00 EUR
Supplier

Details

Target
Calpain 1
Background
The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
Concentration
N/A
Molecular weight
110 kDa
Synonym
Calpain 1
Other name
Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain
NCBI gene number
397027
NCBI gene
CAPN1
Gene source
Porcine
NCBI number
P35750
Recombinant
No
Source
Porcine Erythrocytes
Purification
≥85%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
N/A
Biological activity
≥ 1000 units/mg protein prior to freezing.
Reesults
≥ 1000 units/mg
Binding ability
N/A
Unit
One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 minute at 25°C using the Calpain Activity Assay Kit, (K240-100).
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Liquid
Physical properties
In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
How to reconstitute
N/A
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans