Target of this antibody:
Alternative names of antibody target:
O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylguanine DNA methyltransferase , Methylated DNA protein cysteine methyltransferase
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair protein that response to DNA damage by removing methly or alkyl groups from the O6 position of guanine. MGMT protects normal cells from cytotoxic effects and is important in drμg resistance to alkylating agents.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide corresponding to residues surrounding amino acids 8 of rat MGMT
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-MGMT polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.