Target of this antibody:
Alternative names of antibody target:
ANGPTL4 , ANGPTL-4 , UNQ171/PRO197 , FIAF , ARP4 , ANGPTL2 , Fasting- Induced Adipose Factor , NL2 , pp1158 , PSEC0166 , HFARP
Adipose tissue is an energy reserve in animals. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as adiponectin leptin. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies sμggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 84 of mouse FIAF
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). It recognizes ~50 kDa FIAF from samples of mouse and rat origins.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.