FGF-basic 147, human recombinant

Catalog number
4036-10
Name
FGF-basic 147, human recombinant
Size
10 μg
Price
142.00 EUR
Supplier

Details

Target
FGF-basic 147
Background
Fibroblast Growth Factors, FGFs, are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154) is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, and having a molecular mass of 16.5 kDa.
Concentration
N/A
Molecular weight
16.5 kDa
Synonym
FGFB
Other name
FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2
NCBI gene number
2247
NCBI gene
FGF2
Gene source
Human
NCBI number
P09038
Recombinant
Yes
Source
E. coli
Purification
≥97%
Tested applications
SDS-PAGE, HPLC
Tested activity
N/A
Biological activity
EC50 is typically 0.67-1.45 ng/ml. The activity was determined by the dose-dependent stimulation of mouse BALB/c 3T3 cells.
Reesults
0.67-1.45 ng/ml
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel pack
Physical appearance
Lyophilized protein
Physical properties
Lyophilized from a 10 mM Na2PO4, pH 8.0.
How to reconstitute
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Aa sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants