Target of this antibody:
Alternative names of antibody target:
TPR, Translocated Promoter Region
The vertebrate nuclear pore complex (NPC) is a macromolecular assembly of protein subcomplexes forming a structure of eightfold radial symmetry. The NPC core consists of globular subunits sandwiched between two coaxial ring-like structures of which the ring facing the nuclear interior is capped by a fibrous structure called the nuclear basket. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. TPR localizes to intranuclear filaments of the NPC, and is a component of the cytoplasmic fibrils of the NPC implicated in nuclear protein import. Experimental data suggest that TPR is tethered to intranuclear filaments of the NPC by its coiled coil domain leaving the acidic COOH terminus free to interact with soluble transport factors and mediate export of macromolecules from the nucleus.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human TPR
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. HeLa and 293T cell lysates can be used as positive controls. The antibody recognizes the TPR of human origin. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.