Annexin V-EGFP Reagent

Catalog number
1004-200
Name
Annexin V-EGFP Reagent
Size
200 assays
Price
403.00 EUR
Supplier

Details

Highlights
•Detection method- flow cytometry (Ex/ Em- 488 /530 nm) using FITC signal detector (usually FL1) and fluorescence microscopy •Sample- Cell culture •Simple, easy to use and convenient
Description
The bright and photo-stable reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca²ᶧ -dependent affinity for PS and therefore serves as a probe for detecting apoptosis. The annexin V-EGFP fusion is brighter and photo-stable, ideal for detecting apoptosis by fluorescence microscopy with a FITC filter or by flow cytometry.
Category
Annexin V Apoptosis Assay
Storage condition
+4°C
Shipping condition
gel pack
Maximum time for storage
12 months
Additional description
This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.
Conjugate
The Green Fluorescent Protein (eGFP) of Annexin V- Reagent can be excited at 488 nanometers with a peak emission at 509 nanometers and is detected in the FL1 detector on the FACSCalibur or FACScan. The LSRII and all of the core's sorter flow cytometers can tell the difference between at the same time/together expressing eGFP and eYFP cells when the proper optical filters and experimental controls exist.