Mouse Monoclonal Anti-PPP1A Antibody (1C11-C10-H5-E9)

Catalog number
A1328-100
Name
Mouse Monoclonal Anti-PPP1A Antibody (1C11-C10-H5-E9)
Size
100 µg
Price
338.00 EUR
Supplier

Details

Description
Mouse Monoclonal antibody to detect PPP1A
Raised in
Mouse
Clonality
Monoclonal Antibody (Clone#1C11-C10-H5-E9)
Reacts with
Human
Immunogen
Recombinant human PPP1A protein expressed in E.coli
Target
PPP1A (Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A )
Research area
Signal Transduction
Gene number
5499
Protein number
P53095
Physical state
Colorless liquid
Package form
Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Purity
Affinity purified
Antibody's positive controles
Western blot: MCF7, K562, Hela, HEPG2 and Jurkat whole cell lysates; Immunofluorescence: HeLa cells; Immunohistochemistry: human bresat cancer tissue
Approved applications with antibody dilution
Western blot; 1:1000; Immunofluorescence; 1:300; Immunohistochemistry; 1:500- 11000
Scientific context
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Delivery
Shipped to your lab under gel pack
Precautions
Manifactured in USA. For Research Use Only! Not to be used in humans
Released
This antibody is available to order since May 2017
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus
French translation
anticorps