Histone H3 Peptide, biotin conjugate

Catalog number
7143-01
Name
Histone H3 Peptide, biotin conjugate
Size
100 µg
Price
240.00 EUR
Supplier

Details

Highlights
SOURCE: Synthetic PURITY: ≥ 95% by HPLC MOLECULAR WEIGHT: 2.7643 kDa FORM: Lyophilized powder FORMULATION: Peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in 1 ml/mg dH2O. Avoid freezing and thawing cycles. Store at -20°C for 6 months. KEY APPLICATIONS: The suggested applications include Immunoprecipitation, affinity precipitation, and as substrate for histone modified enzymes, etc. AMINO ACID SEQUENCE: One letter code: ARTKQTARKSTGGKAPR-K(Ac)-QLA-GGK(Biotin)-NH2 Three letter code: H - Ala - Arg - Thr - Lys - Gln - Thr - Ala - Arg - Lys - Ser - Thr - Gly - Gly - Lys - Ala - Pro - Arg - Lys(Ac) - Gln - Leu - Ala - Gly - Gly - Lys(Biotin) - NH2
Description
This peptide is Histone H3 amino acid residues 1-21. It is acetylated at lysine 18 with a C-terminal GG linker followed by a biotinylated lysine. The acetylation of histone H3 at lysine 18 is used to predict prostate cancer recurrence and clinical outcome of patients with both lung and kidney cancer.
Category
Histones
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation
Biotinylated
Additional description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.