Jurkat cell Lysate (untreated)

Catalog number
2401-100
Name
Jurkat cell Lysate (untreated)
Size
100 μg
Price
153.00 EUR
Supplier

Details

Highlights
•Concentration- 2 mg/ml in 1X SDS sample buffer containing 5% β-mercaptoethanol. •Application- The Jurkat cell lysate can be boiled for 5 minutes and then loaded onto SDS-PAGE for Western blotting. 20 µg/lane is recommended for mini gel.
Description
The Jurkat cell lysate was prepared by homogenization in modified RIPA buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. The cell lysate was then diluted to 1X SDS sample buffer (final concentration- 50 mM Tris-HCl, pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol).
Category
Control Lysate
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Additional description
For cells, cell lines and tissues in culture till half confluency.