Target of this antibody:
Alternative names of antibody target:
CAP2 , PI8 , SERPINB8
High molecular weight serine protease inhibitors (serpins) are large superfamily of proteins, which bind to and inactivate serine proteinases. In 1995 Sprecher et al. cloned 2 novel serpins, termed PI8 and PI9, from human placenta cDNA library. Sequencing showed that PI8 encodes 376-amino acids. PI8 are localized to the cytoplasm of transfected cells and was able to form an SDS- complex. PI8 is implicated to inhibit furin enzymatic activity and plays a role in platelet-regulated pathophysiological responses.
Clonality of antibody:
This antibody reacts with:
Synthetic peptide surrounding amino acid 10 of human PI-8
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
0.5 mg/ml purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 1% BSA, 30% glycerol and 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 40-45 kDa human PI-8. Reactivity to other species has not been tested.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.