Target of this antibody:
Alternative names of antibody target:
Bcl-xL, Bcl-xS, BCL2L1, BCL2-like 1 protein
Bcl-xL is a 30 kDa anti-apoptotic protein belonging to the Bcl-2 family. Bcl-xL prevents apoptosis throμgh two different mechanisms: by heterodimerization with an apoptotic protein to inhibit its apoptotic effect, and by its direct pore-forming effect on the outer-membrane of mitochondria to help maintain a normal membrane state under stress conditions. Bcl-xL can be cleaved by caspases upon apoptotic activation, which converts Bcl-xL from an antiapoptotic protein to a proapoptotic cleavage product. The cleavage product of Bcl-xL forms cytochrome c-releasing pores in the mitochondrial membrane to facilitate the apoptosis signaling pathway.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 56 of human Bcl-xL
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified, rabbit anti-Bcl-xL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Western blotting (0.5-4 µg/ml) and immunoprecipitation (20 µ/ml). However, the optimal conditions should be determined individually. The antibody detects the 30 kDa Bcl-xL protein. It does not react with other Bcl-2 family members.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.