Caspase-6, human recombinant

Catalog number
1086-25
Name
Caspase-6, human recombinant
Size
25 units
Price
262.00 EUR
Supplier

Details

Target
Caspase-6
Background
Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli. The active caspase-6 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VEID-pNA (Cat. #1070-200) or Ac-VEID-AFC (1069-200).
Concentration
N/A
Molecular weight
large (18 kDa) and small (11 kDa)
Synonym
Caspase-6
Other name
Caspase-6, CASP-6, Apoptotic protease Mch-2
NCBI gene number
839
NCBI gene
CASP6
Gene source
Human
NCBI number
P55212
Recombinant
Yes
Source
E. coli.
Purification
≥95%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
N/A
Tested activity
> 13000 units/mg
Biological activity
N/A
Reesults
N/A
Binding ability
N/A
Unit
One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Storage condition
-70°C
Shipping under
gel pack
Physical appearance
Lyophilized powder
Physical properties
Lyophilized powder
How to reconstitute
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
Aa sequence
N/A
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants