Ammonium sulfate saturated solution

Catalog number
7096-1L
Name
Ammonium sulfate saturated solution
Size
1 liter
Price
208.00 EUR
Supplier

Details

Highlights
Convenient, ready to use Sterile Ammonium sulfate at 4.32 M. Can be used for: • Purification/Fractionation of proteins • Concentration of protein solutions • Buffer exchange • Remove interference of small molecules from protein/enzyme solutions. Compatible with: • Serum, plasma & other body fluids • Tissues extracts, animal, plant, bacterial extracts, etc. • Cell culture supernatants • Fermentation media
Description
Protein solubility is dependent on the ionic strength of the solution, among other factors. Two distinct phenomenon of protein solubilization are seen in general: 1) Salting in: At low salt concentrations, the solubility of the protein increases with increasing salt concentration (i.e. increasing ionic strength). 2) Salting out: As the salt concentration (ionic strength) increases, the solubility of the protein begins to decrease. At certain ionic strength, the protein will be almost completely precipitate from the solution. Since proteins differ markedly in their solubility at high ionic strength, salting-out is a very useful procedure to assist in the purification of a given protein. Ammonium sulfate has been widely used in salting out for protein purification, as it is very water soluble, forms two ions high in the Hofmeister series1, and has no adverse effects upon enzyme activity. It is generally used as a saturated aqueous solution which is diluted to the required concentration and expressed as a percentage concentration of the saturated solution (a 100% solution). While ammonium sulfate fractionation is a low resolution technique, it is used successfully to reduce the complexity and decrease the sample volume on a daily basis by many scientists.
Category
Protein purification
Storage condition
RT
Shipping condition
RT
Maximum time for storage
24 months
Additional description
The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.