Target of this antibody:
Alternative names of antibody target:
PDE3B, phosphodiesterase type-3
The phosphodiesterase type-3 (PDE3) family is comprised of 2 isoforms, PDE3A and PDE3B. Phosphodiesterases (PDE) are crucial in the regulation of the intracellular level of the second messenger cAMP by hydrolyzing cAMP to 5’AMP. PDE3B has two splice variants that are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium. Both PDE3A and PDE3B are potential targets for the treatment of obesity.
Clonality of antibody:
This antibody reacts with:
Human, mouse, rat
Synthetic peptide surrounding amino acid 948 of mouse PDE3B.
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
200 µg (0.5 mg/ml) affinity purified rabbit anti-PDE3B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
This antibody has been succesfully tested for use in following methods:
The antibody can be used for Western blotting (1–4 µg/ml).
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.