anti-Caspase-10 Antibody

Catalog number
3410-200
Name
anti-Caspase-10 Antibody
Size
Caspase-10 Antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
Caspase-10
Alternative names of antibody target:
CASP10 , ALPS2 , MCH4 , FLICE2 ,CASP-10 , ICE-like apoptotic protease 4
Background information:
Caspase family of cysteine proteases play a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines, and has been identified as caspase-10/a (short form) and caspase-10/b (long form), respectively. Similar as other caspases, caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Activated caspase-10 activates caspase-3,-4,-6,-7,-8, and –9, which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown involving in the Fas and TNFR1 induced apoptosis.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide surrounding amino acid 404 of human caspase-10
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
200 µg (200 µg/ml) affinity purified rabbit anti-caspase-10 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the proform (~55 kDa), intermediates (30 kDa and 34 kDa), and cleaved form (~20 kDa) of caspase-10 on SDS-PAGE immunoblots.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps