anti-PARP Antibody (Clone 10H)

PARP

PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Mouse

Monoclonal

Mouse IgG3κ

10H

All species

1.0 mg/ml

Liquid

1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.

Chromatography

Western blot, Immunoprecipitation, Immunocytochemistry & ELISA

Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods;

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps