anti-PARP Antibody

PARP

PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide corresponding to caspase cleavage site in human PARP

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit anti-PARP polyclonal antibody in phosphatebuffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

Western blot analysis (1-4 µg/ml). The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP. However, the optimal concentrations should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps