anti-FADD Antibody (Clone T1B)

FADD

FAS-associated death domain protein, GIG3, MGC8528, MORT1

Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 24 kDa cytosolic protein. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that can be inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death.

Mouse

Monoclonal

Mouse IgG1

T1B

Human, mouse, dog

Recombinant human FADD

0.2 mg/ml

Liquid

100 µg (200 µg/ml) affinity purified antibody in PBS containing 30% glycerol, 0.5% BSA and 0.01% thimerosal

Affinity purified

Western blot, Immunofluorescence

Western blot analysis (1-4 µg/ml). The antibody detects 28 kDa protein, corresponding to the apparent molecular weight of FADD in SDS-PAGE However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control.immunoblots from samples of human and mouse origins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps